Immunochemical Investigations of Human Pituitary Growth Hormone
نویسندگان
چکیده
منابع مشابه
Immunochemical investigations of human pituitary growth hormone.
Earlier immunological investigations of growth hormone (somatotropin) isolated from bovine and human pituitaries showed that these two protein hormone preparations form speciesspecific antibodies, as evidenced by the results of precipitin ring tests with rabbit antiserum and of anaphylactic shock experiments in guinea pigs (1, 2). It was further demonstrated that the rabbit antiserum exerts in ...
متن کاملPreliminary Investigations on the Action of Pepsin on Human Pituitary Growth Hormone
A slight modification of the original procedure for the isolation of human pituitary growth hormone has been described. The purity of the preparation was further examined by chromatography on carboxymethylcellulose, zone electrophoresis on starch, and electrodialysis. The hormone was found to be stable in a pH 7.0 solution at 100 degrees C and in 10 M urea at 25 degrees C. Digestion with pepsin...
متن کاملState of human growth hormone in plasma and changes in stored solutions of pituitary growth hormone.
Radioimmunoassay of endogenous plasma growth hormone in human plasma fractions separated by starch gel electrophoresis shows that the plasma hormone migrates in a fairly narrow peak behind serum albumin. A similar electrophoretic mobility is exhibited by certain pituitary growth hormone preparations (the A type of Wilhehni), added to plasma in physiological concentrations or on electrophoresis ...
متن کاملThe Amino Acid Composition of Human Pituitary Growth Hormone
The amino acid content in human hypophyseal growth hormone has been determined by chromatography on resin columns. On the basis of 29,000 for the molecular weight, the empirical formula of the hormone was obtained: lys(13)his(5)arg(14)asp(27) thr(14)ser(23)glu(34)pro(12)gly(13)ala(12)-(1/2 cys)(6)val(12)met(4)-ileu(10)leu(31)tyr(10)phe(15)try(1)(NH(3))(32).
متن کاملHuman fetal pituitary expresses functional growth hormone-releasing peptide receptors.
The synthetic hexapeptide GH-releasing peptide (GHRP) stimulates a dose-dependent release of GH in humans in vivo and in animals both in vitro and in vivo via a specific receptor in the hypothalamus and pituitary. To determine the action of GHRP in the human fetal pituitary, reverse transcription-PCR was performed, and GHRP receptor messenger ribonucleic acid expression was detected in fetal pi...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69475-x